Friday, August 21, 2020

Comparing Pursuit of Perfection by Poe and Hawthorne and the Realism of

Quest for Perfection by Poe and Hawthorne and the Realism of Melville and Jacobs  â â â One of the components of Romanticism is the quest for flawlessness. While Poe and Hawthorne's characters endeavor futile for the ideal lady (or rather her ideal characteristic) or the consummately designed individual, Melville definitely realizes that flawlessness is a hallucination. Melville paints a progressively practical representation of the flaws of society. The ladies essayists take Melville's appraisals of the world and the human condition much further. Phelps and Jacobs' know direct about the misguided judgments of flawlessness and the failure to catch that picture. The weight of consistent home life wears on the ladies in these accounts. Jacobs' story worries about the heaviest concern of all being sabotaged by the restraint of ladies and the hardships of servitude.  â â In Poe's Ligeia the storyteller is charmed by his significant other's magnificence and knowledge, with which he gets fixated. He is especially pulled in to the dear music of her low sweet voice. Her uncommon and gigantic learning makes her one of a kind and captivating. Notwithstanding, on the grounds that her insight was, for example, the storyteller had never known in a lady she is a danger. Johanyak says that, Poe's scholarly courageous women are first romanticized and afterward dreaded or misjudged by men who neglect to comprehend or acknowledge their mission for information (63).â The storyteller concedes that he had never known her to blame. Generally, he is yielding that she was in certainty the ideal lady. In the pivotal example of Poe's female characters, such flawlessness must be rebuffed. She bites the dust and the storyteller obsesses about his misfortune. It isn't until this retelling of their marriage that the storyteller really acknowledges all that she was and all that ... ... Dayan, Joan. The Identity of Berenice. Studies in Romanticism 23.4 (1984) 491-513. Holly, Carol. Disgracing the Self in The Angel Over the Right Shoulder. American Literature 60.1 (1988): 42-60. Johanyak, Debra. Poesian Feminism: Triumph or Tragedy. CLA Journal 39.1 (1995): 62-70. Morgan, Winifred. Sex Related Differences in the Slave Narratives of Harriet Jacobs and Frederick Douglass. American Studies 35.2 (1994): 73-94. Rosenberg, Liz. The Best that Earth Could Offer. The Birth-Mark: a Newlywed's Story. Studies in Short Fiction 30.2 (1993): 145-51. Rowland, Beryl. Staying up with a Corpse: Malthus According to Melville in Poor Man's Pudding and Rich Man's Crumbs. Journal of American Studies 6 (1972): 69-83. Zanger, Jules. Talking about the Unspeakable: Hawthorne's The Birth-Mark. Modern Philology 80.4 (1983): 364-71.â

Monday, July 13, 2020

Learn How Effective Drug Addiction Treatment Is

Learn How Effective Drug Addiction Treatment Is Addiction Coping and Recovery Overcoming Addiction Print Learn How Effective Drug Addiction Treatment Is By Buddy T facebook twitter Buddy T is an anonymous writer and founding member of the Online Al-Anon Outreach Committee with decades of experience writing about alcoholism. Learn about our editorial policy Buddy T Medically reviewed by Medically reviewed by Steven Gans, MD on August 05, 2016 Steven Gans, MD is board-certified in psychiatry and is an active supervisor, teacher, and mentor at Massachusetts General Hospital. Learn about our Medical Review Board Steven Gans, MD Updated on July 17, 2018 fstop123 / Getty Images More in Addiction Coping and Recovery Overcoming Addiction Methods and Support Personal Stories Alcohol Use Addictive Behaviors Drug Use Nicotine Use In most cases, the goal of treatment for drug addiction or abuse is not only to get the person to stop using drugs but to also return the individual to a productive member of society. The goal of drug treatment is to not only to stop compulsive drug seeking and use but to help the patient become a functioning member of the family, more capable of becoming employed and improve his or her medical condition. Depending on the circumstances, the goal of drug treatment may also include reducing the criminal behavior of the addict. Effective Drug Treatments According to the Office of National Drug Control Policy, effective drug treatment should, at the very least, lead to the following outcomes: Reduced use of primary drugImprove employment functioningImproved educational statusImproved interpersonal relationshipsImproved general health and medical statusImproved legal statusImproved mental health status Therefore, to measure the effectiveness of drug treatment programs, the rate of abstinence is not the only factor to be considered, but also the functioning of the patient at home, work and in the community. According to the National Institute on Drug Abuse, research shows that overall drug treatment is as successful as treatment of other chronic diseases, such as asthma, diabetes, and high blood pressure. Results of Drug Treatment Programs NIDA research shows that drug treatment programs can produce the following results: Reduce drug use by 40% to 60%Reduce arrests for criminal acts by 40%Methadone treatment reduces criminal behavior by 50%Reduces the risk of HIV infection.Increases the prospects of employment by 40% But, the NIDA is quick to point out that individual treatment outcomes can vary widely depending on many factors, including the patients presenting problems, how well the treatment program addresses those problems and the extent to which the patient is actively involved in the treatment process. The Office of National Drug Control Policys Treatment Protocol Effectiveness Study found that drug treatment programs work when those who abuse drugs can be engaged and retained in treatment and when other needed services can be integrated with drug treatment itself and delivered to help clients resolve the range of problems that accompany their drug use. The 9 Best Online Therapy Programs

Wednesday, May 20, 2020

Gender Stereotypes And Attitudes On Gender Essay - 2043 Words

Gender stereotypes is perceptions and attitudes in which society utilizes to evaluate bias and negative characteristics, positions, roles, and capabilities of males or females. Gender stereotypes create pressure for both men and women. Expectations from society create limitations and often form a gap between men and women by the difference in perceptions, evaluations, and behaviors. In today’s supposedly civilized society, we are induced not to discriminate people. However, society has high expectations on people to act a certain type of behavior. The behavior is based on gender. For example, men are supposed to convey leadership and portray an active figure. On the other hand, women are supposed to be passive and are often valued for their physical appearance. There are numerous articles that discuss how society is able to manipulate the behaviors of others. Above all, it is not that people are obliged or forced into stereotyping females or males, people are just too infatuat ed with generalizing and being judgmental about gender to make themselves feel superior. No matter where, when, what is the environment interact, we all encounter gender stereotypes. Gender stereotypes are present in all cultures, such that the most common distinction are between men and women and in a lot of cases are strictly applied to one gender only, often referring to women. Women are expected to be inferior to men and fragile. This expectation has dominated women s reputation, which limits theShow MoreRelatedGender Stereotypes And Attitudes On Gender Essay2037 Words   |  9 PagesGender stereotypes are perceptions and attitudes in which society utilizes to evaluate bias and negative characteristics, positions, roles, and capabilities of males or females. Gender stereotypes create pressure for both men and women. Expectations from society create limitations and often form a gap between men and women by the difference in perceptions, evaluations, and behaviors. In today’s supposedly civilized society, we are induced not to discriminate people. However, soci ety has high expectationsRead MoreGender Roles And Gender Role Essay784 Words   |  4 Pagesthis session, I will discuss the gender roles in my family. The definition of gender role is the degree to which a person adopts the gender-specific behaviors ascribed by his or her culture (Matsumoto, D. R., Juang 2013, 156). For example, traditional gender roles recommend that males are aggressive, angry, and unemotional. It goes further and explains that the male should leave the home every day to make a living and be the main wage earner. The traditional gender role for the female purpose isRead MoreGender Stereotypes And The Socialization Process1257 Words   |  6 PagesGender Stereotypes and the Socialization Process The pursuit of gender equality is a central element of a vision of sustainability in which every Member of Society respects others and plays a role that allows you to take advantage of their potential to the fullest. The broad goal of gender equality is a social goal that education and other social institutions should contribute. Gender discrimination is embed in the fabric of societies. In many societies, women bear the main burden of food productionRead MoreVisual images Reinforce Traditional Gender and Sexuality Stereotypes948 Words   |  4 Pagesimages reinforce traditional gender and sexuality stereotypes through the manifestation of the masculine and feminine miens. An examination of print media advertisements highlights the social and cultural ideologies associated with traditional gender roles that are expected and imposed on by society. â€Å"Advertisements are deeply woven into the fabric of Western Culture, drawing on and reinforcing commonly held perceptions and beliefs† of gender and sexuality stereotypes. They have a strong role inRead MorePride Vs. Humanity : Jane And Mark840 Words   |  4 Pagesme portray some gender roles. We discussed some roles these past classes, we only cover chapter 1 through 6 but hoping to finish through chapter 17. Chapters 1 through 17 would be the only concern for this essay. There was a number of questions prepared for us to discuss in our essays. I’m going towards the genders roles, there are 4 questions that I will cover to help me interpret this novel. The questions are what is the attitude toward gender roles? , who represents which gender? , what symbolismRead MoreGirls And Girls Were Treated Differently896 Words   |  4 Pagesfamily. Boys and girls were treated differently. We were raised and learned the behaviors and gender role that were acceptable in o ur family and society. It was passing down from generation from generation; we learned what expectations from us as boys and girls are. We tend to get comfortable with the familiar and our routines. There were definitely things boys could do, but not girls. Most of all that gender equity was not for us. Not only inequality in social relationships, women and girls are beingRead MoreHow Women Are Portrayed On Advertising And The Second Looks At This Time1104 Words   |  5 Pagesrole of humor in gender stereotypes. The first one is the role of humor seen in television advertisements and the second looks at peoples attitudes toward humorous and non-humorous ads that have gender stereotypes in them and how that would affect a persons desire to buy. There has been a lot of research done on the gender stereotyping, especially on advertisement and the affect it has on society. The results of previous research find that gender stereotypes reinforce stereotypes in society leadingRead MoreA Research Study On Early Childhood Education1353 Words   |  6 PagesIn early childhood education, young children are starting to learn and understand the concept of gender. Preschool is the age when they start to develop and shape their views regarding gender roles. The population of children who are en rolled in early learning programs are being influenced by their parents, teachers, and classmates, as well as the learning environment they learn, play, and grow in. I analyzed two different research studies, one quantitative and one qualitative, to compare and contrastRead MoreGender Roles In Modern Society728 Words   |  3 PagesGender is an integral part of social practices of human interaction. It influences on all aspects of a persons life such as self-assertion and self-development, family, work, school, etc. Through the gender lens a person perceives any kind of information and passes it to the surrounding world by using a gender display. The process of gender socialization and the content of gender stereotypes has great importance not only for the life of an individual, but also for the progressive development ofRead MoreGender Stereotypes1012 Words   |  5 PagesDevelopment of gender stereotypes in children at home and school There are a lot of parents and teachers who communicate gender stereotypes to children while they interact. We also see how parents interact with boys and girls differently based on their gender. For example, some parents want their children to know that anything with pink is for girls, and blue is for boys. They observe what their parents can do at home, how and in what level their parents communicate to each other, what kind

Wednesday, May 6, 2020

Martin Luther King s Letter From Birmingham Jail

Aristotle, a famous philosopher once said: â€Å" It is absurd to hold that a man ought to be ashamed of being unable to defend himself with his limbs but not of being unable to defend himself with speech and reason, when the use of reason is more distinctive of a human being than the use of his limbs† (Aristotle). He believed that rhetoric was more powerful than fists would ever be. Another man, a very influential social activist, shared the same ideals, preaching to his people to fight for their freedom with words and not their fists. Martin Luther King Jr. used the power of rhetoric during the civil rights movement to gain equality for the black community. MLK was a master of rhetoric and used his knowledge of proper arguments to sway the†¦show more content†¦The Roman Empire fell in 476 C.E. after being conquered by Odoacer, a Germanic leader. Part of the reason the Roman Empire fell was their mistreatment of Christians, who, towards the end of the empire, made up a large portion of the Roman population. King reveals his supposition that the Jim Crow laws and the current racist society will fall away. This also brings into question whether he is trying to evoke guilt among the clergymen. When he compares the persecution of Christians to that of blacks, he is reminding the clergymen that their people were also discriminated against at one point in history, which exposes a hypocrisy where the persecuted become the persecutors. He demonstrates that he has a superior understanding of the values of Christianity and their history by exposing incongruencies within the church as well as their hypocrisy. Describing the devotion of Christians when they truly lived for god instead of for society, MLK demonstrates a complex interpretation of not only the past but current society. He marvels at a time when â€Å"Christians rejoiced at being deemed worthy to suffer for what they believed† and â€Å"the church was not merely† a vessel that â €Å"recorded the ideas and principles of popular opinions† but a catalyst that â€Å"transformed the mores of society† (King). King expresses disappointment that the church has become nothing moreShow MoreRelatedMartin Luther King s Letter From Birmingham Jail1690 Words   |  7 Pages1960’s were a time of civil disobedience and protest against the inequalities in America. Specifically, in Birmingham, Alabama, in the year 1962, Martin Luther King Jr. spoke out against the racial inequalities in one of the most famous letters in America’s history: â€Å"Letter from Birmingham Jail.† This letter was sent to a group of Alabama clergymen who chastised King’s disobedience. The letter was written with the purpose to encourage these eight men to side with King. Martin Luther King Jr.’s knowledgeRead MoreMartin Luther King s Letter From Birmingham Jail1860 Words   |  8 Pagesdisobedience as identified by Martin Luther King is a form of di rect action, and an outright refusal to conform to laws as a form of protest. Martin Luther King addresses this method of resistance in his text, â€Å"Letter from Birmingham Jail†. Martin Luther King Jr personally drafted the text as a response to criticisms made in a statement by clergymen whom apposed King’s nonviolent methods of resistance to continuous issues of racism occurring in Birmingham, Alabama. As expressed by King and described in hisRead MoreMartin Luther King s Letter From Birmingham Jail998 Words   |  4 PagesMartin Luther King, Jr. seldom had time to answer his critics. But on April 16, 1963, he was confined to the Birmingham jail, imprisoned for participating in civil rights demonstrations. â€Å"Alone for days in the dull monotony of a narrow jail cell,† King pondered a letter titled A Call for Unity that fellow clergymen had published pressing him to drop his crusade of nonviolent resistance and to leave the battle for racial equality to the courts. Within that document, King’s fellow clergymen caste himRead MoreMartin Luther King s Letter From Birmingham Jail1562 Words   |  7 PagesDr. Martin Luther King, one of the most influential men in the world, had played a pivotal role during the Civil Rights movement. He led the entire nation in the fight to end segregation, but while trying, he faced many obstacles, one being getting arrested during a rally. While in jail, King had time to respond to the critics of his work in the movement, and he wrote a marvelous, captivating response. In King’s â€Å"Letter from Birmingham Jail,† he replied and prof essed his emotions to the white clergymenRead MoreMartin Luther King s Letter From The Birmingham Jail939 Words   |  4 PagesMartin Luther King preached the realities of what justice truly is. Nearly 50 years later our world is still having trouble with these ideas. There is no doubt that Martin Luther King was an extremely virtuous man. He won the Nobel Peace Prize in 1964, had a Ph.D. in systematic theology, was a baptist minister, and was probably the most recognized person in the civil rights movement. He was assassinated in 1968. The incident as described by Thich Nhat Hanh; I couldn t believe it. I thought theRead MoreMartin Luther King Jr. s Letter From A Birmingham Jail1157 Words   |  5 PagesMartin Luther King Jr.’s â€Å"Letter from a Birmingham Jail† is directed towards the clergymen, although America is his audience, King had come to Birmingham to address the segregation problem in the United States. He refuses to stay silent, even though people told him to wait for the change to happen. King is a part of the Southern Christian Leadership Conference that has many organizations across the South. He felt that he has a right to be in Birmingham because his organizations have connections withRead MoreAnalysis Of Martin Luther King s Letter From Birmingham Jail955 Words   |  4 PagesBy 1963, when Martin Luther King planned a campaign against segregation in Birmingham, Alabama. During the demonstration he was arrested and lives in the jail for eight days. While he was in prison, he wrote his letter from Birmingham Jail to explain his actions and those who urged him to call off the demonstrations. Martin Luther King Jr. Birmingham Jail is important because, he explains the reasons for the non-violent demonstrations, he shows that black people are intelligent, and he criticizesRead MoreMartin Luther King Jr. s Letter From Birmingham Jail934 Words   |  4 PagesIn Martin Luther King Jr.’s , â€Å" Letter from Birmingham Jail †, King responds to the judgments of a group of clergymen , after King s arrest, by writing a letter explaining why the clergymen s judgments were wrong. In his letter, king brings very reasonable and valid points that challenge the judgments of the ministers. The main arguments that king makes would be the reason of his existence in Birmingham, white power structure and its racial injustice, and finally why negotiation has brought up impatienceRead MoreMartin Luther King Jr. s Letter From Birmingham Jail1223 Words   |  5 PagesMartin Luther King, Jr.’s, â€Å"Letter from Birmingham Jail,† emphasizes the need for civil disobedience when faced with unjust laws. This idea contradicts Socrates’ claim made in Crito, that one must follow the law under all circumstances. In this paper, I will argue that Socrates is not a proponent of civil disobedience based on King’s definition of civil disobedience and Socrates’ charges. Moreover, I will ar gue that both Socrates and King disagree with one another based on the concept of civilRead MoreAnalysis Of Martin Luther King s Letter From Birmingham Jail1037 Words   |  5 PagesEssay Four Topic: Read Martin Luther King’s â€Å"Letter from Birmingham Jail† (in your Nexus book) and Brent Staples’s â€Å"Black Men and Public Space.† What do these works say about racism, prejudice, and bias? Have we made any progress in these areas since their publication? What problems do you still see and what solutions can you offer? According to Opposing Viewpoints Online, Racism is â€Å"a prejudice or an animosity against a person or group of people who belong to a different race†. Many Americans wrongly Martin Luther King s Letter From Birmingham Jail The 1960’s were a time of civil disobedience and protest against the inequalities in America. Specifically, in Birmingham, Alabama, in the year 1962, Martin Luther King Jr. spoke out against the racial inequalities in one of the most famous letters in America’s history: â€Å"Letter from Birmingham Jail.† This letter was sent to a group of Alabama clergymen who chastised King’s disobedience. The letter was written with the purpose to encourage these eight men to side with King. Martin Luther King Jr.’s knowledge of Christianity allowed him to successfully sway the clergy. King compared himself to Jesus, several famous people throughout history, and then condemns the clergy, using ethos based in religion. Emotions rooted in deep religion are the†¦show more content†¦Jesus died for the sake of freedom, and Kind allowed himself to be jailed for a similar cause. He chose prison, just as Jesus chose death, for the greater good; he is reminding t he clergy that he is simply following in Christ’s footsteps -- thereby not only guilting them, but also encouraging them to join his movement. While Jesus is the most influential person these clergy could hope to be, King does not fail to use other famous, and holy, people to his advantage. Amos, Paul the Disciple, Martin Luther, and John Bunyan all have two things in common; every man listed is a preacher of some sort, and they were all named by King. He states in his letter â€Å"Was not Amos an extremist for justice: Let justice roll down like waters and righteousness like an ever flowing stream. Was not Paul an extremist for the Christian gospel: I bear in my body the marks of the Lord Jesus. Was not Martin Luther an extremist: Here I stand; I cannot do otherwise, so help me God. And John Bunyan: I will stay in jail to the end of my days before I make a butchery of my conscience.† (King Paragraph 24). While this statement may not initially look like much, upon closer inspection it becomes clear that King is listing people he knows that the clergy also will have religious ties to. Amos has his own book in the bible, Paul was close to Jesus, Martin Luther was a famous pastor, and John Bunyan was a priest, andShow MoreRelatedMartin Luther King s Letter From Birm ingham Jail1860 Words   |  8 Pagesdisobedience as identified by Martin Luther King is a form of direct action, and an outright refusal to conform to laws as a form of protest. Martin Luther King addresses this method of resistance in his text, â€Å"Letter from Birmingham Jail†. Martin Luther King Jr personally drafted the text as a response to criticisms made in a statement by clergymen whom apposed King’s nonviolent methods of resistance to continuous issues of racism occurring in Birmingham, Alabama. As expressed by King and described in hisRead MoreMartin Luther King s Letter From Birmingham Jail998 Words   |  4 PagesMartin Luther King, Jr. seldom had time to answer his critics. But on April 16, 1963, he was confined to the Birmingham jail, imprisoned for participating in civil rights demonstrations. â€Å"Alone for days in the dull monotony of a narrow jail cell,† King pondered a letter titled A Call for Unity that fellow clergymen had published pressing him to drop his crusade of nonviolent resistance and to leave the battle for racial equality to the courts. Within that document, King’s fellow clergymen caste himRead MoreMartin Luther King s Letter From Birmingham Jail1562 Words   |  7 PagesDr. Martin Luther King, one of the most influential men in the world, had played a pivotal role during the Civil Rights movement. He led the entire nation in the fight to end segregation, but while trying, he faced many obstacles, one being getting arrested during a rally. While in jail, King had time to respond to the critics of his work in the movement, and he wrote a marvelous, captivating response. In King’s â€Å"Letter from Birmingham Jail,† he replied and prof essed his emotions to the white clergymenRead MoreMartin Luther King s Letter From The Birmingham Jail939 Words   |  4 PagesMartin Luther King preached the realities of what justice truly is. Nearly 50 years later our world is still having trouble with these ideas. There is no doubt that Martin Luther King was an extremely virtuous man. He won the Nobel Peace Prize in 1964, had a Ph.D. in systematic theology, was a baptist minister, and was probably the most recognized person in the civil rights movement. He was assassinated in 1968. The incident as described by Thich Nhat Hanh; I couldn t believe it. I thought theRead MoreMartin Luther King s Letter From Birmingham Jail1509 Words   |  7 Pagespeople to fight for their freedom with words and not their fists. Martin Luther King Jr. used the power of rhetoric during the civil rights movement to gain equality for the black community. MLK was a master of rhetoric and used his knowledge of proper arguments to sway the opinions of people in power to get what he wanted. Even though Martin Luther King includes an abundance of rhetorical devices in the â€Å"Letter from Birmingham Jail,† he most effectively utilizes ethos and logos, elevating his perceivedRead MoreMartin Luther King Jr. s Letter From A Birmingham Jail1157 Words   |  5 PagesMartin Luther King Jr.’s â€Å"Letter from a Birmingham Jail† is directed towards the clergymen, although America is his audience, King had come to Birmingham to address the segregation problem in the United States. He refuses to stay silent, even though people told him to wait for the change to happen. King is a part of the Southern Christian Leadership Conference that has many organizations across the South. He felt that he has a right to be in Birmingham because his organizations have connections withRead MoreAnalysis Of Martin Luther King s Letter From Birmingham Jail955 Words   |  4 PagesBy 1963, when Martin Luther King planned a campaign aga inst segregation in Birmingham, Alabama. During the demonstration he was arrested and lives in the jail for eight days. While he was in prison, he wrote his letter from Birmingham Jail to explain his actions and those who urged him to call off the demonstrations. Martin Luther King Jr. Birmingham Jail is important because, he explains the reasons for the non-violent demonstrations, he shows that black people are intelligent, and he criticizesRead MoreMartin Luther King Jr. s Letter From Birmingham Jail934 Words   |  4 PagesIn Martin Luther King Jr.’s , â€Å" Letter from Birmingham Jail†, King responds to the judgments of a group of clergymen , after King s arrest, by writing a letter explaining why the clergymen s judgments were wrong. In his letter, king brings very reasonable and valid points that challenge the judgments of the ministers. The main arguments that king makes would be the reason of his existence in Birmingham, white power structure and its racial i njustice, and finally why negotiation has brought up impatienceRead MoreMartin Luther King Jr. s Letter From Birmingham Jail1223 Words   |  5 PagesMartin Luther King, Jr.’s, â€Å"Letter from Birmingham Jail,† emphasizes the need for civil disobedience when faced with unjust laws. This idea contradicts Socrates’ claim made in Crito, that one must follow the law under all circumstances. In this paper, I will argue that Socrates is not a proponent of civil disobedience based on King’s definition of civil disobedience and Socrates’ charges. Moreover, I will argue that both Socrates and King disagree with one another based on the concept of civilRead MoreAnalysis Of Martin Luther King s Letter From Birmingham Jail1037 Words   |  5 PagesEssay Four Topic: Read Martin Luther King’s â€Å"Letter from Birmingham Jail† (in your Nexus book) and Brent Staples’s â€Å"Black Men and Public Space.† What do these works say about racism, prejudice, and bias? Have we made any progress in these areas since their publication? What problems do you still see and what solutions can you offer? According to Opposing Viewpoints Online, Racism is â€Å"a prejudice or an animosity against a person or group of people who belong to a different race†. Many Americans wrongly

The Secret Circle The Divide Chapter 18 Free Essays

Cassie always dreamed of having a sister, of having someone to confide in and share secrets with, someone who would always stick by her no matter what. Cassie and Diana had promised to be sisters to each other, the sisters neither of them had. But that wasn’t going so well these days, or at least not like they thought it would. We will write a custom essay sample on The Secret Circle: The Divide Chapter 18 or any similar topic only for you Order Now But now she had this, a real sister. Well, a half sister, but still, Scarlett was real. Cassie wasn’t an only child anymore. That night, Cassie invited Scarlett to sleep over at her house. She had this urge to learn everything she could about her as soon as possible. Not to gril Scarlett on what she knew of their father and the witch hunters, though of course that was important, too, but everything about her. There was plenty of time for Scarlett to share everything she knew about the hunters with the Circle. But tonight was just about them. They deserved that much. Cassie’s mother was away visiting friends in Cape Cod, so the girls had the whole house to themselves. Cassie was relieved, because she wasn’t sure yet how to broach the subject of Scarlett with her mother. How exactly does one even begin such a conversation? â€Å"Mom, the love of your life, who turned out to be evil? He also had another child.† Especially with a mother like Cassie’s, who would always rather hide from the past and pretend it doesn’t exist. Her mother would stick her head in the sand like an ostrich and live that way forever if she could. Finding out Cassie had a half sister, and worse, that her husband had another daughter with a different woman, might be more than she could handle. It would take a lot of groundwork on Cassie’s behalf to prepare her mother for such a shock. But for tonight, they could just be sisters. She felt herself slip into an immediate playfulness, as if she and Scarlett were trying to make up for the childhood they had been robbed of sharing. For the first few hours, they did all the traditional sleepover things. They ordered a pepperoni pizza and laughed too loud. They painted each other’s nails with sparkled purple nail polish and wolfed down chocolate ice-cream sundaes till they had stomachaches. Then they both changed into their pajamas, and Scarlett pinned Cassie’s hair back in two intertwined French braids. Cassie brushed through Scarlett’s long waves of wild red hair and couldn’t help but ask, â€Å"If you didn’t dye your hair, would it be the same color as mine?† â€Å"Yup,† Scarlett said. â€Å"Look at our eyebrows; they’re the same shade of brown.† â€Å"And our noses are shaped the same.† â€Å"That’s true,† Scarlett said. â€Å"We both have the same perfect button noses.† â€Å"Do you hate peas?† Cassie asked ridiculously. â€Å"I do, but I don’t think that’s genetic.† â€Å"You don’t understand.† Cassie was giggling uncontrollably. â€Å"I hate peas so much, I swear it’s in my DNA.† Scarlett cracked up. Having a sleepover with Scarlett was nothing at all like having one with Diana. Diana always behaved like a serious adult. She rarely loosened up enough to just be silly. But sill iness was no problem for Scarlett. Even though she was a witch, she didn’t always act like one. And even though she’d suffered through intolerable tragedy and loss, she wasn’t mired in somberness. First and foremost, Scarlett was a girl who wanted to have a little fun, and that was a much-needed breath of fresh air to Cassie. They stayed up late into the night talking. The outside world grew quiet and sleepy and finally silent while Cassie and Scarlett remained awake sharing stories. And as the hours passed, their conversation drifted into deeper waters. In hushed tones, Scarlett filled Cassie in on many of the gaps in their family history. â€Å"I always sensed I was different,† she said. â€Å"Even before I knew I was a witch.† â€Å"I know what you mean, believe me.† Cassie brought her knees in toward her chest. â€Å"I never felt at home anywhere. I always felt like a freak.† â€Å"And the dreams and nightmares,† Scarlett said. Cassie nodded. â€Å"Mostly the nightmares.† â€Å"And the strange things that would happen every time I got angry.† Scarlett’s voice rose a bit. â€Å"That was really the kicker.† Cassie nodded more rapidly. The similarities between them were uncanny. Cassie wanted to tell Scarlett about the darkness she sometimes felt inside. Not only the bad feelings triggered by certain people, like the new principal, for example, but that other darkness. The one deep down that she could hardly admit to herself existed. Did Scarlett feel that, too? Did she fear there was some sinister piece of Black John lodged in her soul, infecting and clouding it like a cancerous smoker’s lung? But before Cassie could muster up the courage to ask such a question, Scarlett’s round face turned deathly serious. â€Å"And when I first touched hematite,† she said. â€Å"The feeling in my chest was – â€Å" â€Å"I know!† Cassie screamed out. â€Å"Me, too!† â€Å"It’s my working stone,† Scarlett said. â€Å"Mine, too,† Cassie said. Scarlett grinned knowingly, as if she suspected as much. â€Å"It’s a truly rare occurrence, you know. To have hematite as your working stone.† Cassie turned away for a second, feeling ashamed. She had to remind herself that she didn’t have to be embarrassed of her connection to Black John, at least not with Scarlett. Scarlett watched her patiently. â€Å"It’s okay,† she said. â€Å"I know this is a lot to digest.† She does feel it, Cassie thought. Scarlett understood the mortification of Cassie’s deepest secret. Scarlett endured that same crushing darkness dormant inside herself. The air between them momentarily quieted, and Cassie knew this was her chance to ask about their father. â€Å"It’s because of him,† she said. â€Å"That hematite works for both of us. Right?† Scarlett nodded. â€Å"I’d say that’s most likely the reason.† â€Å"Did you know him?† Cassie asked, not having to utter their father’s name. Scarlett shook her head. â€Å"No. But my mom told me stories. Didn’t yours?† Cassie blushed, shamed by her own mother’s shortcomings. â€Å"Not really.† â€Å"Our moms were best friends growing up,† Scarlett said. â€Å"Did you know that?† Cassie searched her memory for any recol ection of her mother talking about old friends, but she came up blank. â€Å"No,† she said, disappointed. â€Å"I don’t know much at all about my mother’s past.† â€Å"Well, our moms were best friends,† Scarlett said, matter-of-factly. â€Å"Until Black John came between them. Your mom stole him from my mom. That’s why my mom left town.† â€Å"I had no idea.† Cassie’s heart fell a little because of this new picture of her mother, but also because she suddenly thought of Diana and Nick, and how she and Adam came between them. Would things ever be the same between them, or were they bound for the same fate? Scarlett noticed a change in Cassie’s disposition. â€Å"Have I upset you?† she asked. â€Å"Maybe I’m saying too much too soon.† â€Å"No, don’t be silly.† Cassie forced herself to relax and to put Adam and the others out of her mind for now. â€Å"I want to know everything. Don’t hold anything back, please.† Scarlett puckered her lips and eyed Cassie skeptically. â€Å"We have our whole lives to catch up with each other, you know. We don’t have to do it all in one night.† It was an amazingly comforting thought. Our whole lives. They could go back to giggling and goofing around, and pick up this seriousness tomorrow. But Cassie had waited for this chance for far too long to let it go any longer. She needed to know the truth, about everything. â€Å"Please tell me more,† she said. â€Å"I can handle it.† â€Å"Okay then.† Scarlett took Cassie’s hand and squeezed it, and when she did, Cassie looked down at their intertwined fingers. It seemed like she could almost see a cord wrapping around their hands, connecting them. Just like the connection between Adam and me, Cassie thought. She and Scarlett were linked. They were fated. It explained everything she felt about Scarlett since the moment she’d set eyes on her, how she was willing to go against the whole Circle to defend her and protect her. If Scarlett saw it, she didn’t mention it. She went on talking as usual, while massaging Cassie’s hand in her own. â€Å"I’ll never forget the day my mother told me I had a sister,† Scarlett said. â€Å"It changed everything for me. I knew one day I’d find you. And see, I was right.† She waited a moment to read Cassie’s expression and then added, â€Å"I don’t understand why your mom never told you.† Cassie suddenly felt herself snap to a new level of awareness. She pulled her hand away. â€Å"Wait a minute. My mother knew about you?† â€Å"Of course she knew.† Scarlett’s voice contained the â€Å"Of course she knew.† Scarlett’s voice contained the slightest hint of outrage. â€Å"They were all still in New Salem when we were born.† Cassie thought back to the conversation she recently had with her mother. How she’d looked deep into her eyes and swore she’d told Cassie the whole truth about her father. I loved that I was all his, and he was all mine, she’d said, but it was a lie. Her mother knew he was with someone else. â€Å"How could my mother not have told me I had a sister?† Cassie said aloud. This was a new divide that had sprung up between her and her mother, and at the moment, it felt insurmountable. Her whole childhood and adolescence had been hindered by lies – that truth had come to light when they first moved to New Salem, and Cassie learned she was a witch. But she’d come to terms with all the covering up her mother had done in hopes of protecting her. Now it occurred to Cassie that even their more recent conversations had been poisoned by deceit. As of this very moment, her mother was still lying to her. Cassie never felt more estranged from her as she did now. â€Å"She should have told you,† Scarlett said. â€Å"I wonder what else she’s kept from you.† Cassie realized Scarlett was absolutely right. If her mom could lie about the existence of a sibling, she could lie about anything. And if she was keeping secrets, Cassie would, too. She decided right then not to tell her mom anything about meeting Scarlett. Her mother didn’t deserve her honesty. She hadn’t earned it. Fortunately, now Cassie had a sister, and everything would be different. Everything would be better. If it had to be just the two of them against the rest of the world, so be it. They would remain inseparable, that was the one and only thing Cassie could feel secure about now. â€Å"Scarlett,† she said, feeling her heart overflow with love and affection, â€Å"now that you’re here, I finally feel like I’m home.† â€Å"Me, too.† Scarlett’s dark eyes shimmered. â€Å"I’ve never been more sure of anything,† she said. â€Å"This is where I belong.† How to cite The Secret Circle: The Divide Chapter 18, Essay examples

Thursday, April 23, 2020

Lab Reports

Report one Introduction The Green Fluorescent Protein Gene that was noted in one of the species of jelly fish known as Aequoreavictori has been known for several decades. The jelly fish obtained from the Northwest of Pacific was used to clone the gene component. Green light is emitted when this gene species is exposed to Ultra Violet (UV) rays.Advertising We will write a custom report sample on Lab Reports – Gene Technology specifically for you for only $16.05 $11/page Learn More Tsien (1998) observes that it is not necessary to spoil the cells before the process of observation and especially when carrying out gene encoding using the GFP gene. This is mainly attributed to the fact that an outstanding green light can be readily emitted in the presence of UV rays. Background The vector components were part and parcel of the bacterial colonies that were used in this experimental procedure. These are the same components that are supposed to emit green pigment even though they are anticipated to remain chloramphenicol resistant. The PCR was then used to amplify the GFP gene used in the experiment. In order to achieve the desired results, oligonucleotide primers were equally used. Moreover, efficiency of the process was gauged using the two applied methods. In other words, it was possible to determine the efficiency of the cloning methods used by comparing the oligonucleotide primers. GFP clones are amplified when PCR are used in the experiment. The pBCKS are then formed as a result of litigation that takes place in the latter procedure. Thereafter, the electroporation process is used to transform the gene components into the E. coli forms. Discussion The morphological distinction of the GFP gene is possible due to its unique features. Moreover, gene spicing enables the process of GFP production bearing in mind that there are different DNA codes for the organism’s genome that usually targets the proteinsin. Hence, the proce ss of producing GFP genes is controlled by both the regulatory sequence and the gene structure. Past studies have revealed that the cells which take part in the experiment usually emit fluorescent pigment when viewed using fluorescence microscopy. The acceptance of resistance towards germs-killing components is demonstrated using the pBCKs and pGFP cells. The growth levels of the antibiotics can be clearly observed through the plates used in the experiment. The observation can be made clearly especially at the end of the incubation period.Advertising Looking for report on biology? Let's see if we can help you! Get your first paper with 15% OFF Learn More Hence, the experiment used and represented the GFP gene as plasmid. It was then placed together with a component derived from DNA. This explains why green colour was emitted when the GFP cells were exposed to UV light. Nonetheless, there were quite a number of regions that failed to emit the green colour. Tsien (1998) also observed the same experience in one of his similar experiments. The regions that do not fluoresce may be composed of the GFP genes. Nevertheless, the transcription, translational, and replication process can be experienced by the cells taking part in the experiment. In contrast, there is evident resistance towards Chloramphenacol among the grown pBCK cells. As a result, the GFP gene expression cannot be observed. This explains why some regions could not fluoresce (emit green light) even when exposed to Ultra Violet rays. The gene expression is imitated and also the present fluorescent protein is utilized by E. coli that has been activated biologically. However, when blue light is passed over the gene component, the green colour is emitted. Wang, et al. (1994) also gave the same conclusions in one of their experiments. Hence, it can then be clearly indicated that the inserted lac promoter component and the GFP gene were part and parcel of the downstream cloning process. The inse rted component was also found to be relatively active in the presence of Escherichia coli. When pBCKS and pGFPgene plasmids were subjected to the restriction, linear vectors were formed. Sticky ends are also produced. These ends are laced with both the TTAA and AATT on the plasmids. The chloramphenicol plates also demonstrated growth of some colonies. Moreover, the antibiotics used received a lot of resistance from the cells used in the experiment. Fluorescent was not produced even after exposure to UV rays. Hence, it indicated that the litigation reaction had failed or become impaired. From the experimental results, it was evident that when the pGFP plasmid was used in the sub cloning process, the process did not succeed. This was mainly occasioned by the fact that there was partial digestion process or the system was inactive. Report two Introduction The field of medicine has immensely benefited from the process of protein localization in E. coli using Green Fluorescent Protein (G FP). One of the areas of concerns has been the development of gene fusions. Two main components used in this process have been the variant of gfp and malE [MBP]. The GFPuv has also been optimized using the latter components. The hybrid proteins used to be encoded using GFP combined with carboxy-terminal end.Advertising We will write a custom report sample on Lab Reports – Gene Technology specifically for you for only $16.05 $11/page Learn More Background The -galactosidase expression is induced using IPTG in the lac promoter region. When this component is used, the involved gene sequence and the performance of the transcription are significantly improved. The BioRad dye binding assay is also used to estimate various quantities of protein components used in the experiment. The process is carried out using specific time intervals. According to this experiment, both the GFP protein and the protein components that were used work in a similar manner. The reagents are then applied with the Gel electrophoresis in order to identify the proteins taking part in the experiment. Hence, its main role is to monitor the levels at which protein quantities are increasing. It is also instrumental in determining the concentration levels of GFP proteins that are present in the induced cultures. Discussion In order to express GFP, the recombinant E. coli can be used in a unique manner. In this case, it is applied as a gene product model component for investigating various conditions in organisms. Cha et al. (2000) is quite categorical that when making use of GFP, a gene in a specific organism can be easily expressed. Moreover, it is possible for the system of an organism to be introduced with the component. It can equally be integrated in the genome system especially through viral vector injection, cell transformation and also when carrying out breeding process. In this experiment, colour change was the main guiding factor for correlating conce ntration and the level of protein assayed component. The color changes were compared with 595 nm. When proteins are absent, the results are blank and the indicator shows a zero mark. When a linear equation y = 0.16x + 0.36 is used, it is possible to obtain the level of concentration of the unknown protein samples. From the results, a standard curve was drawn to indicate the various levels of protein concentration. The results indicated that the GFP concentration was high at 0.5ug/ul. Moreover, there was a remarkable content expression when the protein sample was induced at 0 hours. This was mainly attributed by the either the background expression or basal expression. This finding is also supported by research studies conducted by Tsien (1998). According to this experimental result, the GFP components that are identical to the protein expressions have been facilitated by the E coli component. When optimal expression is analyzed, the best operating conditions were not considered as t he 3 hours that were taken and the lac promoter used. In fact, the contents of the lac promoter affected the final results.Advertising Looking for report on biology? Let's see if we can help you! Get your first paper with 15% OFF Learn More The E coli activities also affected the components of the GFP gene during the process of downstream cloning. Kosinski, Rinas and Bailey (1992) reiterate that when E coli strain is combined with IPTG, it agitates the rate of growth of any given protein. However, the procedure ought to be carried out when lac promoter is present. The electrophoresis processes with the assistance of SDS gel were used to demonstrate the expressed protein purity. Protein bands that were almost same in size were also visualized. The GFP protein size was also demonstrated by the fact that the sizes ranged between 27 and 30 KDa. However, Burnette (1981) notes that the migration rates affect these sizes. Besides, the binding dye effects affected the clarity of the final outcomes. According to Burnette (1981), the generation of coloured precipitates is affected by the primary antibodies. The precipitation levels are also affected when the subsrate are added. Clear bands were seen at 30KDa since the dye bound well with the GFP protein components This report on Lab Reports – Gene Technology was written and submitted by user Stella Horne to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.